Application of RNA-sequencing to identify biomarkers in broiler chickens prophylactic administered with antimicrobial agents

Antimicrobial (AM) resistance is largely acknowledged as one of the biggest global health and food safety challenges overuse AMs known to generate in bacteria that may affect both animals humans. Poultry meat second most-produced European Union recent years consumers are becoming more concerned about safety, traceability, animal welfare poultry rearing system, increasingly requiring meats from broilers reared without AMs. In present study, we performed RNA sequencing analyze 64 liver 54 muscle transcriptomic profiles treatment or treated with different classes Moreover, validated most differentially expressed genes among groups detect putative novel biomarkers able discriminate The PDK4, IGFBP1, RHOB were identified hepatic biomarkers, discriminating compared treatments. whole transcriptome changes revealed a valuable target organ for AM administration screening. addition, our results suggest leading effect coccidiostat when associated AMs, influencing several biological processes. Our study showed powerful method aberrant regulated identify biomarker candidates misuse detection farm animals. Further validation on larger sample size wider spectrum needed confirm viability aforementioned population.